Measurement

Part:BBa_I8510:Design

Designed by: Reshma Shetty   Group: iGEM07_Example   (2008-01-09)


3OC6HSL->Inverter->lacZalpha with orthogonal GFP protein generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2398
    Illegal BamHI site found at 3775
    Illegal XhoI site found at 3826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3278
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2096
    Illegal BsaI.rc site found at 727
    Illegal BsaI.rc site found at 1952


Design Notes

This composite system has several copies of the BBa_B0015 terminator which make make it prone to recombination.


Source

This is a composite system for measuring the behavior of inverter BBa_Q20060 and for verifying that inverter function is specific (the BBa_C2006 repressor specifically represses the BBa_R2000 promoter).

References